| Primary Identifier | MGI:3639337 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Bnc2 |
| Inheritance Mode | Not Specified | Transmission | Germline |
| Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Integration site GenBank ID: AB187235 |
| molecularNote | The "promoter trap" gene trap vector pU21 contains 1.2 kb of intron 1 and part of exon 2, including the splice acceptor, from the mouse engrailed 2 gene (En2) followed by the Betageo beta-galactosidase/neomycin resistance fusion gene with the SV40 and mouse Pgk1 polyadenylation signals. Three stop codons occur in the exon sequence upstream of and in frame with the Betageo translation initiation codon. loxP and FRT sites used for 5' RACE analysis and for in vitro exchange of other sequences for Betageo are present. The gene trap vector inserted into the intron between exon 2a and exon3. The absence of transcript expression was confirmed by RT-PCR. LacZ staining confirmed expression in the embryonic head and around the palatal cleft. |
