| First Author | Haustein MD | Year | 2014 |
| Journal | Neuron | Volume | 82 |
| Issue | 2 | Pages | 413-29 |
| PubMed ID | 24742463 | Mgi Jnum | J:209943 |
| Mgi Id | MGI:5569094 | Doi | 10.1016/j.neuron.2014.02.041 |
| Citation | Haustein MD, et al. (2014) Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron 82(2):413-29 |
| abstractText | The spatiotemporal activities of astrocyte Ca(2)(+) signaling in mature neuronal circuits remain unclear. We used genetically encoded Ca(2)(+) and glutamate indicators as well as pharmacogenetic and electrical control of neurotransmitter release to explore astrocyte activity in the hippocampal mossy fiber pathway. Our data revealed numerous localized, spontaneous Ca(2)(+) signals in astrocyte branches and territories, but these were not driven by neuronal activity or glutamate. Moreover, evoked astrocyte Ca(2)(+) signaling changed linearly with the number of mossy fiber action potentials. Under these settings, astrocyte responses were global, suppressed by neurotransmitter clearance, and mediated by glutamate and GABA. Thus, astrocyte engagement in the fully developed mossy fiber pathway was slow and territorial, contrary to that frequently proposed for astrocytes within microcircuits. We show that astrocyte Ca(2)(+) signaling functionally segregates large volumes of neuropil and that these transients are not suited for responding to, or regulating, single synapses in the mossy fiber pathway. |
