Primary Identifier | MGI:3641275 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cast |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Most of exon 6 was replaced with a pgk-neo in reverse orientation. Northern and Western blot analysis confirmed recombination of the locus, as did RT-PCR. Calpastatin activity assays indicated that calpastatin activity was reduced to an undetectable level in the mutant mouse brain. |