Primary Identifier | MGI:3640123 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Dp(16Dgcr14-Ufd1l)217Bld |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A proximal loxP site was targeted into the Es2el gene. The targeting vector replaced a 200-bp fragment containing exon 4. A second loxP site was targeted into the Ufd1l gene, replacing a 4.5-kb fragment containing exons 2-3. Transient expression of Cre resulted in recombination between loxP sites on opposite chromosomes duplicating the 1.2 MB region from Es2el to Ufd1l. |