| First Author | Wang H | Year | 2007 |
| Journal | Mol Immunol | Volume | 44 |
| Issue | 7 | Pages | 1765-74 |
| PubMed ID | 17007932 | Mgi Jnum | J:116045 |
| Mgi Id | MGI:3692793 | Doi | 10.1016/j.molimm.2006.07.301 |
| Citation | Wang H, et al. (2007) Association of the pre-B cell receptor (BCR) expression level with the quality of pre-BII cell differentiation reveals hierarchical pre-BCR function. Mol Immunol 44(7):1776-85 |
| abstractText | The expression of a pre-B cell receptor (pre-BCR) is required for allelic exclusion and pre-BII cell differentiation. V(H)12 muH chains are unusual in that they form pre-BCRs and mediate allelic exclusion, but most cannot drive pre-BII cell differentiation. To explain this paradox, we examined pre-BCR functions and pre-BII cell differentiation in mice expressing muH chain transgenes encoding a B cell-permissible V(H)12 muH chain (designated 10/G4(6-1)), and a non-permissible V(H)12 muH chain (designated 8/G0). Compared with 10/G4 pre-BCRs, 8/G0 pre-BCRs are expressed at low levels on the cell surface. 8/G0 pre-BCRs mediate allelic exclusion, but 8/G0 pre-BII cells are defective in proliferation and expression of survival factors Bcl-2, Bcl-X(L) and hemokinin 1 (HK1). Increasing 8/G0 muH chain production restores HK1 transcription and improves proliferation of pre-BII cells as well as later stage B cell development. These data reveal a hierarchy of pre-BCR function that determines the development and plasticity of early B cells. |
