| Primary Identifier | MGI:3652575 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(CAG-cat,-EGFP)39Miya |
| Strain of Origin | (C57BL/6NJcl x DBA/2NJcl)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | Of nine transgenic lines whose offspring from crosses with cre transgenic mice fluoresced, four - numbered 20, 39 and 42, with estimated transgene copy numbers of 22, 21 and 13, respectively - were propagated. Line 39 was used in later experiments because of the strong fluorescence of the peripheral lymphocytes of its cre-transgenic progeny. |
| molecularNote | This Cre recombinase reporter transgene employs the "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene. The promoter is separated from the EGFP coding sequence, with a rabbit globin polyadenylation signal, by a loxP-flanked chloramphenicol acetyltransferase gene (cat) with the SV40 small t intron and polyadenylation signal. The cat gene is efficiently excised by Cre recombinase, permitting EGFP expression under control of the beta-actin promoter. |
