| Primary Identifier | MGI:3653502 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Atp2a2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was used to insert an inactivated muscle-specific 5' donor splice site and a strong polyA site. A neomycin resistance cassette replaced untranslated exons 23 and 24, and the muscle-specific exon 25. The resulting allele lacked expression of the protein in the heart and prevented its replacement by the b isoform. RNase protection assay and Western blot failed to detect transcript and protein, respectively, in mutants. |
