Primary Identifier | MGI:3664469 | Allele Type | Targeted |
Gene | Braf | Transmission | Germline |
Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The human ARAF cDNA was introduced in frame with exon 3 of the locus. This exon is common to all three isoforms and harbors the beginning of the Ras binding domain. RT-PCR and Western blot confirmed the production of chimeric transcripts and protein from the locus. A floxed neo included in the vector did not interfere with expression of the protein. |