| First Author | Dieterich LC | Year | 2015 |
| Journal | Cell Rep | Volume | 13 |
| Issue | 7 | Pages | 1493-1504 |
| PubMed ID | 26549461 | Mgi Jnum | J:228958 |
| Mgi Id | MGI:5749897 | Doi | 10.1016/j.celrep.2015.10.002 |
| Citation | Dieterich LC, et al. (2015) DeepCAGE Transcriptomics Reveal an Important Role of the Transcription Factor MAFB in the Lymphatic Endothelium. Cell Rep 13(7):1493-504 |
| abstractText | VEGF-C/VEGFR-3 signaling plays a central role in lymphatic development, regulating the budding of lymphatic progenitor cells from embryonic veins and maintaining the expression of PROX1 during later developmental stages. However, how VEGFR-3 activation translates into target gene expression is still not completely understood. We used cap analysis of gene expression (CAGE) RNA sequencing to characterize the transcriptional changes invoked by VEGF-C in LECs and to identify the transcription factors (TFs) involved. We found that MAFB, a TF involved in differentiation of various cell types, is rapidly induced and activated by VEGF-C. MAFB induced expression of PROX1 as well as other TFs and markers of differentiated LECs, indicating a role in the maintenance of the mature LEC phenotype. Correspondingly, E14.5 Mafb(-/-) embryos showed impaired lymphatic patterning in the skin. This suggests that MAFB is an important TF involved in lymphangiogenesis. |
