| Primary Identifier | MGI:3663426 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Pde10a |
| Transmission | Germline | Strain of Origin | DBA/1LacJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A PGK-neo vector was designed to replace exons 14-16, corresponding to bases 1499-1861 of the coding region. Sequence analysis revealed that the neo cassette was spliced out. The resulting transcript encoded an in-frame deletion of 126 amino acids at the N-terminus of the catalytic domain. This terminus contained one of the two divalent zinc binding sites. Since the deletion was in-frame, a mutant protein of approximately 75 kDa could be produced. |
