| Primary Identifier | MGI:3665254 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Ifng |
| Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An IRES element, eYFP and a bovine polyA sequence followed by a floxed neomycin resistance cassette were inserted into the end of the coding sequence. Targeting took place in ES cells homozygous for Tg(Prm-cre)70Og, which express Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. The protamine-cre transgene was separated from the knock-in allele by crossing germline male mutant mice with C57BL/6 or BALB/c mice. |
