| First Author | Wiles KN | Year | 2021 |
| Journal | Cell Mol Gastroenterol Hepatol | Volume | 12 |
| Issue | 5 | Pages | 1743-1759 |
| PubMed ID | 34311141 | Mgi Jnum | J:313045 |
| Mgi Id | MGI:6793886 | Doi | 10.1016/j.jcmgh.2021.07.007 |
| Citation | Wiles KN, et al. (2021) IkappaB Kinase-beta Regulates Neutrophil Recruitment Through Activation of STAT3 Signaling in the Esophagus. Cell Mol Gastroenterol Hepatol 12(5):1743-1759 |
| abstractText | BACKGROUND & AIMS: The epithelial barrier is the host's first line of defense against damage to the underlying tissue. Upon injury, the epithelium plays a critical role in inflammation. The IkappaB kinase beta (IKKbeta)/nuclear factor-kappaB pathway was shown to be active in the esophageal epithelium of patients with esophageal disease. However, the complex mechanisms by which IKKbeta signaling regulates esophageal disease pathogenesis remain unknown. Our prior work has shown that expression of a constitutively active form of IKKbeta specifically in esophageal epithelia of mice (Ikkbetaca(Esophageal Epithelial Cell-Knockin ()(EEC-KI)())) is sufficient to cause esophagitis. METHODS: We generated ED-L2/Cre;Rosa26-Ikkbetaca(+/L);Stat3(L/L) (Ikkbetaca(EEC-KI);Stat3(Esophageal Epithelial Cell Knockout ()(EEC-KO)())) mice, in which the ED-L2 promoter activates Cre recombinase in the esophageal epithelium, leading to constitutive activation of IKKbeta and loss of Stat3. Esophageal epithelial tissues were collected and analyzed by immunostaining, RNA sequencing, quantitative real-time polymerase chain reaction assays, flow cytometry, and Western blot. Ikkbetaca(EEC-KI) mice were treated with neutralizing antibodies against interleukin (IL)23p19 and IL12p40. RESULTS: Here, we report that Ikkbetaca(EEC-KI) mice have increased activation of epithelial Janus kinase 2/STAT3 signaling. Stat3 deletion in Ikkbetaca(EEC-KI) mice attenuated the neutrophil infiltration observed in Ikkbetaca(EEC-KI) mice and resulted in decreased expression of genes related to immune cell recruitment and activity. Blocking experiments in Ikkbetaca(EEC-KI) mice showed that STAT3 activation and subsequent neutrophil recruitment are dependent on IL23 secretion. CONCLUSIONS: Our study establishes a novel interplay between IKKbeta and STAT3 signaling in epithelial cells of the esophagus, where IKKbeta/IL23/STAT3 signaling controls neutrophil recruitment during the onset of inflammation. GEO accession number: GSE154129. |
