Primary Identifier | MGI:3688272 | Allele Type | Transgenic |
Attribute String | Inserted expressed sequence | Gene | Tg(Tcrb2B4)1Mmd |
Strain of Origin | (C57BL/6J x C3H/HeJ)F2 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The rearranged 2B4 beta chain with 4.5 kb of 5' sequence was cleaved at the EcoRV site in the 3' untranslated exon of Cbeta and a XhoI linker was inserted. The resulting 9.3 kb fragment extending from the 5' end of the clone was ligated to a 3 kb fragment carrying the 3' end of the C beta 2. the immunoglobin heavy-chain enhancer was nserted immediately downstream of the Jbeta2.5 segment. |