| First Author | Coverdell TC | Year | 2022 |
| Journal | Cell Rep | Volume | 39 |
| Issue | 11 | Pages | 110962 |
| PubMed ID | 35705034 | Mgi Jnum | J:333138 |
| Mgi Id | MGI:7294010 | Doi | 10.1016/j.celrep.2022.110962 |
| Citation | Coverdell TC, et al. (2022) Genetic encoding of an esophageal motor circuit. Cell Rep 39(11):110962 |
| abstractText | Motor control of the striated esophagus originates in the nucleus ambiguus (nAmb), a vagal motor nucleus that also contains upper airway motor neurons and parasympathetic preganglionic neurons for the heart and lungs. We disambiguate nAmb neurons based on their genome-wide expression profiles, efferent circuitry, and ability to control esophageal muscles. Our single-cell RNA sequencing analysis predicts three molecularly distinct nAmb neuron subtypes and annotates them by subtype-specific marker genes: Crhr2, Vipr2, and Adcyap1. Mapping the axon projections of the nAmb neuron subtypes reveals that Crhr2(nAmb) neurons innervate the esophagus, raising the possibility that they control esophageal muscle function. Accordingly, focal optogenetic stimulation of cholinergic Crhr2(+) fibers in the esophagus results in contractions. Activating Crhr2(nAmb) neurons has no effect on heart rate, a key parasympathetic function of the nAmb, whereas activating all of the nAmb neurons robustly suppresses heart rate. Together, these results reveal a genetically defined circuit for motor control of the esophagus. |
