| First Author | Scaldaferri ML | Year | 2015 |
| Journal | Mech Dev | Volume | 136 |
| Pages | 53-63 | PubMed ID | 25684074 |
| Mgi Jnum | J:292669 | Mgi Id | MGI:6436741 |
| Doi | 10.1016/j.mod.2015.02.002 | Citation | Scaldaferri ML, et al. (2015) Hematopoietic activity in putative mouse primordial germ cell populations. Mech Dev 136:53-63 |
| abstractText | In the present paper, starting from the observation of heterogeneous expression of the GOF-18DeltaPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFP(weak) CD-34(weak/high) cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation. |
