| First Author | Valente M | Year | 2023 |
| Journal | Nat Immunol | Volume | 24 |
| Issue | 4 | Pages | 714-728 |
| PubMed ID | 36928414 | Mgi Jnum | J:337732 |
| Mgi Id | MGI:7495983 | Doi | 10.1038/s41590-023-01454-9 |
| Citation | Valente M, et al. (2023) Novel mouse models based on intersectional genetics to identify and characterize plasmacytoid dendritic cells. Nat Immunol 24(4):714-728 |
| abstractText | Plasmacytoid dendritic cells (pDCs) are the main source of type I interferon (IFN-I) during viral infections. Their other functions are debated, due to a lack of tools to identify and target them in vivo without affecting pDC-like cells and transitional DCs (tDCs), which harbor overlapping phenotypes and transcriptomes but a higher efficacy for T cell activation. In the present report, we present a reporter mouse, pDC-Tom, designed through intersectional genetics based on unique Siglech and Pacsin1 coexpression in pDCs. The pDC-Tom mice specifically tagged pDCs and, on breeding with Zbtb46(GFP) mice, enabled transcriptomic profiling of all splenic DC types, unraveling diverging activation of pDC-like cells versus tDCs during a viral infection. The pDC-Tom mice also revealed initially similar but later divergent microanatomical relocation of splenic IFN(+) versus IFN(-) pDCs during infection. The mouse models and specific gene modules we report here will be useful to delineate the physiological functions of pDCs versus other DC types. |
