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Publication : A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs.

First Author  Dolt KS Year  2013
Journal  PLoS One Volume  8
Issue  4 Pages  e62054
PubMed ID  23637962 Mgi Jnum  J:200557
Mgi Id  MGI:5508851 Doi  10.1371/journal.pone.0062054
Citation  Dolt KS, et al. (2013) A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs. PLoS One 8(4):e62054
abstractText  There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models.
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