| Primary Identifier | MGI:3694735 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Slc5a2-cre)1Tauc |
| Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The transgene expresses Cre recombinase under control of the promoter region of the gene encoding solute carrier family 5 (sodium/glucose cotransporter), member 2, including 1952 bp of 5'-flanking sequence, the first exon (containing a mutated translation initiation codon), first intron and 23 bp of the second exon. X-gal staining of multiple tissues from mice doubly transgenic for this transgene and for the E. coli beta-galactosidase gene (lacZ) preceded by a loxP-flanked stop sequence (knocked into the Gt(ROSA)26Sor locus) demonstrated beta-galactosidase activity only in the kidney. Immunohistochemical analysis of X-gal-stained kidney cryostat sections for markers specific to different regions of the nephron showed colocalization of X-gal staining only with anti-megalin antibody, which identifies the proximal tubules. |
