| Primary Identifier | MGI:3697168 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Camk2a-cre)20Kmik |
| Strain of Origin | Not Specified | Is Recombinase | true |
| Is Wild Type | false |
| description | Of several transgenic lines created, lines 10 and 26 exhibit higher Cre recombinase expression than lines 17N, 11 and 20. |
| molecularNote | Expression of bacteriophage P1 Cre recombinase, bearing the nuclear localization signal peptide of SV40 large T antigen, is directed by the promoter of the mouse gene encoding calcium/calmodulin-dependent protein kinase II alpha. Expression of the transgene was demonstrated by RT-PCR analysis, immunoblot analysis and a reporter assay for Cre recombinase activity. Activity is present specifically in the nuclear fraction of the cerebrum, although a low level (1-10% of that in the cerebrum) of "leaky" expression is observed in the cerebellum as well. |
