| First Author | Liu Y | Year | 2018 |
| Journal | Bone | Volume | 111 |
| Pages | 28-35 | PubMed ID | 29530719 |
| Mgi Jnum | J:267524 | Mgi Id | MGI:6199716 |
| Doi | 10.1016/j.bone.2018.03.009 | Citation | Liu Y, et al. (2018) Rescue of a cherubism bone marrow stromal culture phenotype by reducing TGFbeta signaling. Bone 111:28-35 |
| abstractText | We utilized a bone marrow stromal culture system to investigate changes in TGFbeta signaling in a mouse model for cherubism (Sh3bp2(KI/KI)). Interestingly, bone marrow cultures derived from cherubism mice not only displayed impaired osteoblast differentiation, but also had spontaneous osteoclast formation. PAI1, a target gene of TGFbeta signaling, was elevated 2-fold in cherubism CD11b(-),CD45(-) cells compared to wild type cells, while the expression of BAMBI, an inhibitor of TGFbeta signaling, was down-regulated. We also discovered that treatment of cherubism cultures with antagonists of the TGFbeta signaling pathway could largely rescue osteoblast differentiation and markedly reduce spontaneous osteoclast formation. Treatment with the type I TGFbeta receptor small molecule inhibitor SB505124 increased osteoblast reporter gene Col1a1-2.3 expression 24-fold and increased the expression of osteoblast gene markers Osterix (Sp7) 25-fold, Bone Sialoprotein (BSP) 7-fold, Osteocalcin (Bglap1) 100-fold, and Dentin Matrix Protein 1 (DMP1) 35-fold. In contrast, SB505124 treatment resulted in a significant reductions in osteoclast number and size. Gene expression analyses for RANKL, a positive regulator of osteoclast formation was 2.5-fold higher in osteoblast cultures derived from Sh3bp2(KI/KI) mice compared to wild type cultures, whereas OPG, an inhibitor of RANKL was 5-fold lower. However, SB505124 treatment reduced RANKL almost back down to wild type levels, while increasing OPG expression. Our studies also implicate a role for TGFbeta ligands in the etiology of cherubism. Blocking of TGFbeta ligands with the monoclonal antibody 1D11 increased Col1a1-2.3 reporter expression 4-fold and 13-fold in cultures derived from Sh3bp2(KI/+) and Sh3bp2(KI/KI) mice, respectively. Serum levels of latent TGFbeta1 were also 2-fold higher in SH3BP2(KI/KI) mice compared to wild type littermates. Taken together, these studies provide evidence that elevated levels of TGFbeta signaling may contribute to the disease phenotype of cherubism and a reduction in pathway activity may be an effective therapeutic approach to treat this rare disease. |
