| First Author | Shi L | Year | 2023 |
| Journal | Redox Biol | Volume | 63 |
| Pages | 102727 | PubMed ID | 37156218 |
| Mgi Jnum | J:335860 | Mgi Id | MGI:7483818 |
| Doi | 10.1016/j.redox.2023.102727 | Citation | Shi L, et al. (2023) FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgfbeta1 signaling pathway. Redox Biol 63:102727 |
| abstractText | Adipose plasticity is critical for metabolic homeostasis. Adipocyte transdifferentiation plays an important role in adipose plasticity, but the molecular mechanism of transdifferentiation remains incompletely understood. Here we show that the transcription factor FoxO1 regulates adipose transdifferentiation by mediating Tgfbeta1 signaling pathway. Tgfbeta1 treatment induced whitening phenotype in beige adipocytes, reducing UCP1 and mitochondrial capacity and enlarging lipid droplets. Deletion of adipose FoxO1 (adO1KO) dampened Tgfbeta1 signaling by downregulating Tgfbr2 and Smad3 and induced browning of adipose tissue in mice, increasing UCP1 and mitochondrial content and activating metabolic pathways. Silencing FoxO1 also abolished the whitening effect of Tgfbeta1 on beige adipocytes. The adO1KO mice exhibited a significantly higher energy expenditure, lower fat mass, and smaller adipocytes than the control mice. The browning phenotype in adO1KO mice was associated with an increased iron content in adipose tissue, concurrent with upregulation of proteins that facilitate iron uptake (DMT1 and TfR1) and iron import into mitochondria (Mfrn1). Analysis of hepatic and serum iron along with hepatic iron-regulatory proteins (ferritin and ferroportin) in the adO1KO mice revealed an adipose tissue-liver crosstalk that meets the increased iron requirement for adipose browning. The FoxO1-Tgfbeta1 signaling cascade also underlay adipose browning induced by beta3-AR agonist CL316243. Our study provides the first evidence of a FoxO1-Tgfbeta1 axis in the regulation of adipose browning-whitening transdifferentiation and iron influx, which sheds light on the compromised adipose plasticity in conditions of dysregulated FoxO1 and Tgfbeta1 signaling. |
