| Primary Identifier | MGI:3701804 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Prokr2 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The splice acceptor of exon 3 was duplicated along with 147 bp of coding region and fused to a Hprt containing vector creating a null allele. Targeted mutagenesis was confirmed by RT-PCR for the presence of exons 1, 2 and 3 but the absence of an expressed RNA segment between exons 2 and 3. Radiolabeled ligand (mamba intestinal toxin 1) was also used to confirm the absence of functional protein. |
