| Primary Identifier | MGI:3701508 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Igh |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT-flanked neomycin resistance cassette was inserted in reverse orientation just downstream of the DQ52 region in a plasmid containing DQ52 (Igh-D). A heavy chain variable-diversity-joining (VDJ) segment of HyHEL10 sequences were placed downstream of the neomycin cassette, replacing the Igh-J (Jh1-4) locus. A loxP site was added to a downstream SpeI restriction site, between the Igh-6 (IgM/mu) enhancer and the Igh-6 switch region, for use in future gene targeting. The immunoglobulin heavy chain locus is of the Igha allotype. |
