| First Author | Serrano Martinez P | Year | 2022 |
| Journal | iScience | Volume | 25 |
| Issue | 10 | Pages | 105047 |
| PubMed ID | 36147959 | Mgi Jnum | J:328932 |
| Mgi Id | MGI:7341203 | Doi | 10.1016/j.isci.2022.105047 |
| Citation | Serrano Martinez P, et al. (2022) Role of quiescent cells in the homeostatic maintenance of the adult submandibular salivary gland. iScience 25(10):105047 |
| abstractText | Stem/progenitor cells are required for maintenance of salivary gland (SG) function and serve as untapped reservoirs to create functional cells. Despite recent advancements in the identification of stem/progenitor pools, in the submandibular gland (SMG), a knowledge gap remains. Furthermore, the contribution to adult SMG homeostasis of stem/progenitor cells originating from embryonic development is unclear. Here, we employ an H2B-GFP embryonic and adult pulse-and-chase system to characterize potential SMG stem/progenitor cells (SGSCs) based on quiescence at different stages. Phenotypical profiling of quiescent cells in the SMG revealed that label-retaining cells (LRCs) of embryonic or adult origin co-localized with CK8+ ductal or vimentin + mesenchymal, but not with CK5+ or CK14 + stem/progenitor cells. These SMG LRCs failed to self-renew in vitro while non-label retaining cells displayed differentiation and long-term expansion potential as organoids. Collectively, our data suggest that an active cycling population of cells is responsible for SMG homeostasis with organoid forming potential. |
