| Primary Identifier | MGI:3702333 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neo cassette with a 3' loxP site followed by an alkaline phosphatase gene with a 3' loxP site was inserted into exon 3. Transient cre expression was used to knockin a transgene composed of 1.4kb of the promoter of Penk1, 600bp of the 5' UTR and first intron of Penk1 fused to a LacZ cassette and the 3'UTR of Penk1 between the alkaline phosphatase gene and the second loxP site. RT-PCR confirmed transgene expression of LacZ mRNA in brain, heart and liver tissue. |
