| Primary Identifier | MGI:3702336 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neo cassette with a 3' loxP site followed by an alkaline phosphatase gene with a 3' loxP site was inserted into exon 3. Transient cre expression was used to knockin transgene composed of 5.0kp of promoter region and 600bp of 5'UTR and intron 1 of Penk1 fused to a lacZ cassette along with a portion of the Penk1 3'UTR between the alkaline phosphatase gene and the second loxP site. Expression of the insertion was confirmed by RT-PCR of the brain, heart and liver all of which expressed lacZ mRNA. |