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Allele : Hprt1<tm2(Penk1-lacZ)Saub> hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Brian Sauer

Primary Identifier  MGI:3702336 Allele Type  Targeted
Attribute String  Null/knockout, Reporter Gene  Hprt1
Transmission  Germline Strain of Origin  129S4/SvJae
Is Recombinase  false Is Wild Type  false
molecularNote  A neo cassette with a 3' loxP site followed by an alkaline phosphatase gene with a 3' loxP site was inserted into exon 3. Transient cre expression was used to knockin transgene composed of 5.0kp of promoter region and 600bp of 5'UTR and intron 1 of Penk1 fused to a lacZ cassette along with a portion of the Penk1 3'UTR between the alkaline phosphatase gene and the second loxP site. Expression of the insertion was confirmed by RT-PCR of the brain, heart and liver all of which expressed lacZ mRNA.
  • mutations:
  • Insertion
  • synonyms:
  • ENK5.0,
  • ENK5.0
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

4 Publication categories

Trail: Allele