| Primary Identifier | MGI:3703164 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter | Gene | Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), human TEL-AML1 fusion protein sequence (coding for the translocation t(12;21)(p13;q22)), internal ribosomal entry site, and an enhanced green fluorescent protein (EGFP) gene. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human TEL-AML1 fusion protein and EGFP to proceed in the Cre recombinase expressing cells. |
