Primary Identifier | MGI:3704091 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Dbt |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was used to replace part of exon 4 and all of exon 5 with a PGKneo cassette. Southern blot and immunohistochemical analyses verify the absence of wild-type E2 transcripts and lack of E2 protein detection in homozygotes. |