Primary Identifier | MGI:3707322 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Crmp1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A multi-step targeting process consisting of the insertion and subsequent removal of a floxed HPRT cassette into intron 5, and the insertion of a second floxed HPRT cassette into intron 3 resulted in the flanking of exons 4-5 with loxP sites. Transient cre expression ultimately excised both exons and the second HPRT cassette. Northern blot and Western blot confirmed absence of transcript and protein, respectively, in mutants. |