| Primary Identifier | MGI:3708000 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sphk2 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT-flanked neo cassette with a single 3' loxP site was inserted between exons 1 and 2 and an additional loxP site was inserted downstream of exon 5. The neo cassette was subsequently removed through flp mediated recombination. In vivo Cre-mediated recombination removed exons 2 through 5 creating a null allele. The loss of transcript was confirmed by RT-PCR. |
