| Primary Identifier | MGI:3709190 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(Npy-hrGFP)1Lowl |
| Strain of Origin | FVB | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence into the translational start site of the mouse neuropeptide Y (Npy) gene. Correctly transformed and targeted bacteria were used to isolate the ~200 kb transgenic construct (called "NPY-GFP"). This NPY-GFP transgene was used for pronuclear injection into fertilized one-cell stage FVB embryos. Founder line 1 showing germline transmission was established. Brain tissues show GFP fluorescence when exposed to UV light. |
