| First Author | Saito K | Year | 2006 |
| Journal | Neuroscience | Pages | abstract |
| Mgi Jnum | J:122360 | Mgi Id | MGI:3714146 |
| Citation | Saito K (2006) Generation and analysis of vesicular GABA transporter knockout mouse. Neuroscience :abstract |
| abstractText | Full text of Abstract: Neuroscience 2006 The 29th Annual Meeting of the Japan Neuroscience Society Program Number: PS1A-C035 Presentation Time: Wednesday, July 19, 11:00-12:00 Generation and analysis of vesicular GABA transporter knockout mouse. Kenzi Saito1,2,3, Kenji Nakamura4, Toshikazu Kakizaki1,3, Satoe Ebihara5, Masakazu Uematsu6, Shigeo Takamori7, Minesuke Yokoyama4, Shiro Konishi4, Masayoshi Mishina3,8, Jun-ichi Miyazaki9, Kunihiko Obata10, Yuchio Yanagawa1,3. 1Gunma Univ., Maebashi, Japan, 2SOKENDAI, Hayama, Japan, 3SORST, Kawaguchi, Japan, 4Mitsubishi Kagaku INST. Life Sci., Machida, Japan, 5Kumamoto Univ., Kumamoto, Japan, 6ToyohashI Univ. Tech., Toyohashi, Japan, 7Tokyo Med. Den. Univ., Tokyo, Japan, 8Univ. Tokyo, Tokyo, Japan, 9Osaka Univ., Suita, Japan, 10RIKEN, Wako, Japan. The vesicular GABA transporter (VGAT) loads GABA from neuronal cytoplasm into synaptic vesicles and is selectively expressed in inhibitory neurons containing GABA and/or glycine. To assess the functional role of VGAT in development, we have disrupted the gene encoding VGAT using Cre-loxP system. Western-blot analysis showed that VGAT protein was absent in the homozygous embryos, indicating that the mutation had generated in a null allele. VGAT knockout mice died around birth. All VGAT knockout mice displayed cleft palate and omphalocele. Our results suggest that VGAT plays essential roles in both palate formation and ventral body wall development. Keywords: VGAT, GABA, INHIBITORY NEURON Research Funds: KAKENHI (17052002) |
