| First Author | Jin HK | Year | 1998 |
| Journal | Biochem Genet | Volume | 36 |
| Issue | 9-10 | Pages | 311-22 |
| PubMed ID | 9919357 | Mgi Jnum | J:52028 |
| Mgi Id | MGI:1327718 | Doi | 10.1023/a:1018741312058 |
| Citation | Jin HK, et al. (1998) Characterization and expression of the Mx1 gene in wild mouse species. Biochem Genet 36(9-10):311-22 |
| abstractText | The mouse Mx1 gene encodes an interferon (IFN)-inducible nuclear protein and confers resistance to influenza virus infection. The standard laboratory mouse strains all carry the Mx1- allele and are susceptible to influenza virus. In this study, several mouse strains established from wild mice were tested to determine their Mx1+ or Mx1- allele status with polymerase chain reaction-restriction fragment length variation (PCR-RFLV), sequence analysis, reverse transcription (RT)-PCR, and immunofluorescence staining. All of the mouse strains originating from wild mice were found uniformly to carry the Mx1+ allele. Therefore, it is conceivable that the Mx1+ allele in wild populations serves a function against some pathogens related to orthomyxoviruses. The PCR-RFLV and sequence analysis allowed us to classify the Mx1+ alleles of the laboratory and wild-origin mouse strains into distinct classes. RT-PCR and immunofluorescence staining demonstrated that the Mx1 transcripts and proteins were induced by IFN-alpha/beta in macrophages from wild mouse species. |
