| Primary Identifier | MGI:3715453 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Syt5 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to fuse a signal peptide from rat neurexin 1 alpha followed by a GFP "emerald" cassette to the amino(N) terminus of exon 1, flank exon 2 with loxP sites, and insert an Flp-flanked neomycin resistance cassette in intron 2. Resultant mice were crossed with an FRT recombinase strain to remove the FLP-flanked neomycin cassette. Presence of the GFP fusion protein was detected by western blot analysis. |
