| Primary Identifier | MGI:3716991 | Allele Type | Transgenic |
| Attribute String | Transactivator | Gene | Tg(Camk2a-rtTA)1237Kndl |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| description | Two transgenic lines, designated 1237 and 1076, were developed. Data were reported only for the former; authors state that results for line 1076 are similar to those for line 1237. |
| molecularNote | The transgene comprises 8.5 kb of upstream flanking DNA and 85 bp of the 5', noncoding exon of the mouse calcium/calmodulin-dependent protein kinase II alpha gene, an artificial intron and splice signal, the coding sequence for the reverse tetracycline-regulated transactivator (rtTA) protein and an SV40 polyadenylation signal. Maximal doxycycline induction of mice bearing both this transgene and a lacZ reporter transgene under control of the tetracycline operator (tetO) demonstrated expression in the CA1 and CA2 regions, but only minimal expression in the CA3 region, of the hippocampus; in the dentate gyrus; in both superficial layers and a deep layer of the cortex; and in the septum and striatum. |
