| Primary Identifier | MGI:3717392 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Gfap-APOE_i4*L28P)12Hube |
| Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The cDNA encoding human APOE isoform 4 carrying the substitution C112R was mutated at residue 28 from leucine to proline (L28P) by site-specific mutagenesis. The cDNA was cloned into a vector downstream of the rat glial fibrillary acid protein (Gfap) promoter and regulatory elements and upstream of the human beta-globin splice and polyadenylation sites. The linearized construct was injected into the male pronuclei of B6D2F1 zygotes. |
