| First Author | Chen Z | Year | 2017 |
| Journal | Circulation | Volume | 136 |
| Issue | 24 | Pages | 2359-2372 |
| PubMed ID | 29021323 | Mgi Jnum | J:271458 |
| Mgi Id | MGI:6279617 | Doi | 10.1161/CIRCULATIONAHA.117.030137 |
| Citation | Chen Z, et al. (2017) Pathologic Stimulus Determines Lineage Commitment of Cardiac C-kit(+) Cells. Circulation 136(24):2359-2372 |
| abstractText | BACKGROUND: Although cardiac c-kit(+) cells are being tested in clinical trials, the circumstances that determine lineage differentiation of c-kit(+) cells in vivo are unknown. Recent findings suggest that endogenous cardiac c-kit(+) cells rarely contribute cardiomyocytes to the adult heart. We assessed whether various pathological stimuli differentially affect the eventual cell fates of c-kit(+) cells. METHODS: We used single-cell sequencing and genetic lineage tracing of c-kit(+) cells to determine whether various pathological stimuli would result in different fates of c-kit(+) cells. RESULTS: Single-cell sequencing of cardiac CD45(-)c-kit(+) cells showed innate heterogeneity, indicative of the existence of vascular and mesenchymal c-kit(+) cells in normal hearts. Cardiac pressure overload resulted in a modest increase in c-kit-derived cardiomyocytes, with significant increases in the numbers of endothelial cells and fibroblasts. Doxorubicin-induced acute cardiotoxicity did not increase c-kit-derived endothelial cell fates but instead induced cardiomyocyte differentiation. Mechanistically, doxorubicin-induced DNA damage in c-kit(+) cells resulted in expression of p53. Inhibition of p53 blocked cardiomyocyte differentiation in response to doxorubicin, whereas stabilization of p53 was sufficient to increase c-kit-derived cardiomyocyte differentiation. CONCLUSIONS: These results demonstrate that different pathological stimuli induce different cell fates of c-kit(+) cells in vivo. Although the overall rate of cardiomyocyte formation from c-kit(+) cells is still below clinically relevant levels, we show that p53 is central to the ability of c-kit(+) cells to adopt cardiomyocyte fates, which could lead to the development of strategies to preferentially generate cardiomyocytes from c-kit(+) cells. |
