| First Author | Matsu-Ura T | Year | 2016 |
| Journal | Mol Cell | Volume | 64 |
| Issue | 5 | Pages | 900-912 |
| PubMed ID | 27867006 | Mgi Jnum | J:252213 |
| Mgi Id | MGI:6094677 | Doi | 10.1016/j.molcel.2016.10.015 |
| Citation | Matsu-Ura T, et al. (2016) Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. Mol Cell 64(5):900-912 |
| abstractText | Circadian clock-gated cell division cycles are observed from cyanobacteria to mammals via intracellular molecular connections between these two oscillators. Here we demonstrate WNT-mediated intercellular coupling between the cell cycle and circadian clock in 3D murine intestinal organoids (enteroids). The circadian clock gates a population of cells with heterogeneous cell-cycle times that emerge as 12-hr synchronized cell division cycles. Remarkably, we observe reduced-amplitude oscillations of circadian rhythms in intestinal stem cells and progenitor cells, indicating an intercellular signal arising from differentiated cells governing circadian clock-dependent synchronized cell division cycles. Stochastic simulations and experimental validations reveal Paneth cell-secreted WNT as the key intercellular coupling component linking the circadian clock and cell cycle in enteroids. |
