| First Author | Kuwano T | Year | 2021 |
| Journal | PLoS One | Volume | 16 |
| Issue | 3 | Pages | e0248267 |
| PubMed ID | 33760832 | Mgi Jnum | J:304399 |
| Mgi Id | MGI:6690414 | Doi | 10.1371/journal.pone.0248267 |
| Citation | Kuwano T, et al. (2021) Generation and characterization of a Meflin-CreERT2 transgenic line for lineage tracing in white adipose tissue. PLoS One 16(3):e0248267 |
| abstractText | Meflin (Islr) expression has gained attention as a marker for mesenchymal stem cells, but its function remains largely unexplored. Here, we report the generation of Meflin-CreERT2 mice with CreERT2 inserted under the Meflin gene promoter to label Meflin-expressing cells genetically, thereby enabling their lineages to be traced. We found that in adult mice, Meflin-expressing lineage cells were present in adipose tissue stroma and had differentiated into mature adipocytes. These cells constituted Crown-like structures in the adipose tissue of mice after high-fat diet loading. Cold stimulation led to the differentiation of Meflin-expressing lineage cells into beige adipocytes. Thus, the Meflin-CreERT2 mouse line is a useful new tool for visualizing and tracking the lineage of Meflin-expressing cells. |
