| First Author | Sono T | Year | 2020 |
| Journal | Am J Pathol | Volume | 190 |
| Issue | 9 | Pages | 1909-1920 |
| PubMed ID | 32533926 | Mgi Jnum | J:293726 |
| Mgi Id | MGI:6452299 | Doi | 10.1016/j.ajpath.2020.05.017 |
| Citation | Sono T, et al. (2020) Perivascular Fibro-Adipogenic Progenitor Tracing during Post-Traumatic Osteoarthritis. Am J Pathol 190(9):1909-1920 |
| abstractText | Perivascular mural cells surround capillaries and microvessels and have diverse regenerative or fibrotic functions after tissue injury. Subsynovial fibrosis is a well-known pathologic feature of osteoarthritis, yet transgenic animals for use in visualizing perivascular cell contribution to fibrosis during arthritic changes have not been developed. Here, inducible Pdgfra-CreER(T2) reporter mice were subjected to joint-destabilization surgery to induce arthritic changes, and cell lineage was traced over an 8-week period with a focus on the joint-associated fat pad. Results showed that, at baseline, inducible Pdgfra reporter activity highlighted adventitial and, to a lesser extent, pericytic cells within the infrapatellar fat pad. Joint-destabilization surgery was associated with marked fibrosis of the infrapatellar fat pad, accompanied by an expansion of perivascular Pdgfra-expressing cellular descendants, many of which adopted alpha-smooth muscle actin expression. Gene expression analysis of microdissected infrapatellar fat pad confirmed enrichment in membrane-bound green fluorescent protein/Pdgfra-expressing cells, along with a gene signature that corresponded with injury-associated fibro-adipogenic progenitors. Our results highlight dynamic changes in joint-associated perivascular fibro-adipogenic progenitors during osteoarthritis. |
