| Primary Identifier | MGI:3720264 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Gt(ROSA)26Sor-BCHE*G117H)844Loc |
| Strain of Origin | C57BL/6 x (C57BL/6 x SJL)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The human G117H BChE sequence (encoding the 28 amino acid signal peptide and 574 amino acid full-length protein) was modified with a single His for Gly amino acid mutation at codon 117 (G117H). To enhance expression, a chimeric intron (consisting of a human beta-globin gene and the branch and 3' acceptor site from the intron of an immunoglobulin gene heavy chain variable region) was placed between the promoter and human G117H BChE sequence. A FLAG epitope tag (encoding amino acids DYKDDDDK) was placed at the carboxy-terminus of the human G117H BChE. The transgene also contained the human elongation factor 1-alpha poly(A) sequence at the 3' end. This transgene was microinjected into fertilized [C57BL/6 x (C57BL/6xSJL)F1] eggs. Founder mice (line M844T) were bred to B6SJL/F1Tac mice. All tested tissues express G117H BChE enzyme activity. |
