| Primary Identifier | MGI:3722076 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Cyp1a2 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct with a long arm for recombination encompassing 4429 bp of Cyp1a2 genomic sequence plus 48 bp of 3'-flanking sequence was followed by a floxed PGK-NEO gene and then by 1452 bp of 3'-flanking sequence for the short arm. An HSK-TK negative-selection cassette was placed outside the 3' Cyp1a2 homology arm. This construct was electroporated into 129S6/SvEvTac ES cells. In recombined ES cells, an allele containing a floxed PGK-NEO gene, placed 350 bp 3' of the stop codon and a loxP site located 48 bp 3' beyond the end of exon 7 was generated. |
