| Primary Identifier | MGI:3721141 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Nlrp3 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene was disrupted by the insertion of an EGFP cassette fused in frame with the ATG of exon 2. A selection cassette PGK-neo flanked by 2 loxP sites was also inserted in the intron 2. The neo cassette was deleted by backcrossing the mice with a Cre-expressing deleter strain. RT-PCR analysis of RNA isolated from mutant macrophages confirmed that the wild-type transcript is absent. |
