Primary Identifier | MGI:3722875 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Atp2c1 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin selection cassette replaced exons 10-13, which encode both the catalytic phosphorylation domain and transmembrane domains 3 and 4. RT-PCR and sequencing analysis revealed expression of a mutant mRNA in which exon 9 is spliced to exon 14. Western analysis revealed decreased protein expression in heterozygous mice |