| Primary Identifier | MGI:3723291 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Sox10 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was constructed that contains a Sox10 open reading that encodes for a deletion of amino acids 233-306, followed by a loxP flanked neo cassette. The region deleted has been implicated in the transactivation potential of this transcription factor. The vector replaced the entire open reading frame of Sox10 by deleting exons 3 through 5. The neo cassette was removed via in vivo Cre mediated recombination. Quantitative RT-PCR on mid-stage embryos indicated that mRNA levels of the mutated gene were similar to the wild-type gene. |
