| Primary Identifier | MGI:3757650 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Arhgef9 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Transfection of ES cells with a cre construct was used to remove exon 5 along with an inserted neo cassette. Exon 5 encodes part of the catalytic RhoGEF domain. Its deletion and resulting frameshift are predicted to destroy the function of the protein and eliminate the entire C-terminus. The absence of full length and truncated protein products was confirmed by western blot on brain homogenates using either a monoclonal specific for the N-terminus or polyclonal antibody against Arhgef9. |
