| First Author | Guan Y | Year | 2020 |
| Journal | Sci Adv | Volume | 6 |
| Issue | 13 | Pages | eaaz2129 |
| PubMed ID | 32232159 | Mgi Jnum | J:289957 |
| Mgi Id | MGI:6435245 | Doi | 10.1126/sciadv.aaz2129 |
| Citation | Guan Y, et al. (2020) SKP1 drives the prophase I to metaphase I transition during male meiosis. Sci Adv 6(13):eaaz2129 |
| abstractText | The meiotic prophase I to metaphase I (PI/MI) transition requires chromosome desynapsis and metaphase competence acquisition. However, control of these major meiotic events is poorly understood. Here, we identify an essential role for SKP1, a core subunit of the SKP1-Cullin-F-box (SCF) ubiquitin E3 ligase, in the PI/MI transition. SKP1 localizes to synapsed chromosome axes and evicts HORMAD proteins from these regions in meiotic spermatocytes. SKP1-deficient spermatocytes display premature desynapsis, precocious pachytene exit, loss of PLK1 and BUB1 at centromeres, but persistence of HORMAD, gammaH2AX, RPA2, and MLH1 in diplonema. Strikingly, SKP1-deficient spermatocytes show sharply reduced MPF activity and fail to enter MI despite treatment with okadaic acid. SKP1-deficient oocytes exhibit desynapsis, chromosome misalignment, and progressive postnatal loss. Therefore, SKP1 maintains synapsis in meiosis of both sexes. Furthermore, our results support a model where SKP1 functions as the long-sought intrinsic metaphase competence factor to orchestrate MI entry during male meiosis. |
