| First Author | Kallurkar PS | Year | 2022 |
| Journal | Sci Rep | Volume | 12 |
| Issue | 1 | Pages | 2923 |
| PubMed ID | 35190626 | Mgi Jnum | J:346554 |
| Mgi Id | MGI:6885091 | Doi | 10.1038/s41598-022-06834-z |
| Citation | Kallurkar PS, et al. (2022) Transcriptomes of electrophysiologically recorded Dbx1-derived respiratory neurons of the preBotzinger complex in neonatal mice. Sci Rep 12(1):2923 |
| abstractText | Breathing depends on interneurons in the preBotzinger complex (preBotC) derived from Dbx1-expressing precursors. Here we investigate whether rhythm- and pattern-generating functions reside in discrete classes of Dbx1 preBotC neurons. In a slice model of breathing with ~ 5 s cycle period, putatively rhythmogenic Type-1 Dbx1 preBotC neurons activate 100-300 ms prior to Type-2 neurons, putatively specialized for output pattern, and 300-500 ms prior to the inspiratory motor output. We sequenced Type-1 and Type-2 transcriptomes and identified differential expression of 123 genes including ionotropic receptors (Gria3, Gabra1) that may explain their preinspiratory activation profiles and Ca(2+) signaling (Cracr2a, Sgk1) involved in inspiratory and sigh bursts. Surprisingly, neuropeptide receptors that influence breathing (e.g., micro-opioid and bombesin-like peptide receptors) were only sparsely expressed, which suggests that cognate peptides and opioid drugs exert their profound effects on a small fraction of the preBotC core. These data in the public domain help explain the neural origins of breathing. |
