| Primary Identifier | MGI:3761144 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ramp1 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele was generated by breeding mice heterozygous for Ramp1tm1Tsuj with transgenic mice ubiquitously expressing cre recombinase. Exon 2 of the gene and the neomycin resistance cassette were removed, leaving behind a single loxP site. Upon deletion of exon 2, the splicing of exons 1 to 3 created a frameshift that resulted in the introduction of a termination codon at the beginning of exon 3. RT-PCR analysis of the thymus indicated there was no transcription of the wild-type gene. |
